Part:BBa_I716026:Design

p-zhuC promoter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

making basic biobrick I716026 - Zinc repressed promoter (p-zhuC)

[pcr] AY019/20 onto MG1655 genome   (251 bp, BglII/XhoI)
put it into pBca9145
you get I716026

AY019 // catggAGATCTggcggataatgctgcgaaaag
AY020 // gtacaCTCGAGttaGGATCCtcaaaatttttccaggttta

Source

K12 MG1655